Hordein Gene Expression in a Low Protein Barley Cultivar

Hordein gene expression in a low protein barley cultivar.

The low protein barley (Hordeum vulgare L.) cultivar ;Karl' is deficient in hordeins, the major storage protein fraction, but has normal levels of other endosperm protein fractions. We compared hordein gene expression in Karl with that in ;Traill,' a related cultivar that contains normal amounts of hordein. In plants grown in controlled environmental conditions, hordein accumulation in Karl was...

Antioxidative activity of barley hordein and its loss by deamidation.

Barley hordein was comparable to maize zein in antioxidation under a powder model system. Various deamidated "hordein" preparations were obtained and examined for their molecular-size distribution (by Sephacryl S-100 gel filtration), hydrophobicity (by fluorescence measurement using fluorescent probes) and antioxidative activity (by the ferric thiocyanate method). Deamidation caused fragmentati...

HORDEIN GENE DOSE EFFECTS IN TRIPLOID ENDOSPERM OF BARLEY (Hordeum vulgare L.)

Cloning and Expression of the Coat Protein Gene of Barley Yellow Dwarf Virus-PAV in Escherichia coli

Low copy number gene transfer and stable expression in a commercial wheat cultivar via particle bombardment.

Two groups of linear gene constructs (gus and bar, and 1Ax1 and bar) lacking vector backbone sequences were independently transferred into the elite wheat (Triticum aestivum L.) variety EM12, and genetically stable transgenic plants with low copy number transgene integration were recovered. Co-transformation experiments were carried out in parallel using either circular whole plasmid(s) or line...